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| 黄粉虫防御性分泌物抑菌活性的研究 | |
| 引用本文： | 强承魁,杨兆芬,杜予州,檀东飞,郑福山.黄粉虫防御性分泌物抑菌活性的研究[J].生物技术,2006,16(1):22-24. |
| 作者姓名： | 强承魁 杨兆芬 杜予州 檀东飞 郑福山 |
| 作者单位： | 1. 扬州大学应用昆虫研究所,江苏,扬州,225009;福建师范大学生物工程学院,福建,福州,350007 2. 福建师范大学生物工程学院,福建,福州,350007 3. 扬州大学应用昆虫研究所,江苏,扬州,225009 |
| 基金项目： | 福建省教育厅基金项目资助(No.JB01104) |
| 摘 要： | 目的:研究黄粉虫防御性分泌物的化学成分及其抑菌活性。方法:用二氯甲烷萃取分泌物并经GC/MS分析,后用牛津杯法和平板连续稀释法分别就该分泌物对大肠杆菌、金黄色葡萄球菌、白色假丝酵母、黑曲霉、桔青霉的抑菌作用及2-甲基对苯醌和对甲酚标准品对上述供试菌的最低抑菌浓度进行测定和比较。结果:分泌物含2-甲基对苯醌、对甲酚和正二十三烷等7种成分,抑菌强度:桔青霉>大肠杆菌和金黄色葡萄球菌>白色假丝酵母>黑曲霉。结论:该分泌物对这5种供试菌的抑制效果优于2-甲基对苯醌和对甲酚标准品。 |
| 关 键 词： | 黄粉虫 防御性分泌物 抑菌活性 GC/MS |
| 文章编号： | 1004-311x（2006）01-0022-03 |
| 收稿时间： | 2005-10-15 |
| 修稿时间： | 2005-12-09 |
Study on the Antimicrobial Activity of the Defensive Secretion of Tenebrio molitor L. |
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| QIANG Cheng-kui,YANG Zhao-fen,DU Yu-zhou,TAN Dong-fei,ZHENG Fu-shan.Study on the Antimicrobial Activity of the Defensive Secretion of Tenebrio molitor L.[J].Biotechnology,2006,16(1):22-24. | |
| Authors: | QIANG Cheng-kui YANG Zhao-fen DU Yu-zhou TAN Dong-fei ZHENG Fu-shan |
| Institution: | 1, Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China; 2, Bioengineering College, Fujian Normal University, Fuzhou 350007, China |
| Abstract: | Objective:To study on the chemical components and the antimicrobial activities of the defensive secretion of Tenebrio molitor L..Methods: The secretion was extracted by dichloromethane and analyzed by gas chromatography/mass spectrometry(GC/MS).Then Oxford Cup method was used in detecting and discussing the antimicrobial activities of the secretion against Escherichia coli,Staphylococcus aureus,Candida albicans,Aspergillus niger and Penicillium citrinum.Dilute-plate method was used to detect and discuss the minimal inhibitory concentration(MIC) of the standard materials of methyl-p-benzoquinone(MBQ) and p-cresol on the tested strains.Results: The secretion included seven kinds of chemicals,such as MBQ,p-cresol,tricosane and so forth.Its inhibitory intensity: Penicillium citrinum>Escherichia coli and Staphylococcus aureus>Candida albicans>Aspergillus niger.Conclusion: The secretion was more effective in inhibiting the growth of the five species of microbes than the standard materials of MBQ and p-cresol. |
| Keywords: | Tenebrio molitor L defensive secretion antimicrobial activity GC/MS |
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