大肠癌P21,P53蛋白表达和k—ras基因,P53基因突变研究

用免疫组化技术和ＰＣＲ－ＳＳＣＰ技术对高、中、低分化大肠腺癌、癌旁粘膜、正常粘膜及大肠腺癌型息肉的Ｐ２１、Ｐ５３蛋白表达和ｋ－ｒａｓ基因、Ｐ５３基因突变进行检测。结果，大肠腺癌Ｐ２１、Ｐ５３蛋白表达比大肠腺瘤增多，但增加不显著（Ｐ〉０．０５），二组均比癌旁粘膜和正常粘膜Ｐ２１、Ｐ５３蛋白表达阳性率高（Ｐ〈０．０１），大肠腺癌ｋ－ｒａｓ基因和Ｐ５３基因突变率比大肠腺瘤、癌旁粘膜和正常粘膜组显著增加

Expression of P21,P53 protein and mutation of k-ras gene, P53 gene in colorectal carcinoma

P21,P53 protein expression and k-ras, P53 gene mutation was detected in well-differentiated, moderately differentiated, poorly differentiated colorectal carcinoma, adjacent mucosa, normal mucosa and adenomatous polyps, with ABC immunohistochemistry and PCR-SSCP silver staining technigue. P21,P53 protein expression in colorectal carcinoma was increased not significantly compared with those in colorectal adenoma, but significantly higher than those in adjacent mucosa and normal mucosa. k-ras gene and P53 gene mutation was found to correlate with P21, P53 protein expression. The poor differentiated in tumor progression was accompanied by P21,P53 portein overexpression and k-ras, P53 gene frequetly mutaion, but the difference is not significant (P>0 05). k-ras gene, P53 gene mutation and P21, P53 protein expression may play an important role during colorectal carcinogenesis.