| 微生物法生产普鲁兰酶的研究 |
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| 引用本文： | 吴燕萍,金其荣.微生物法生产普鲁兰酶的研究[J].生物技术,1998,8(6):14-17. |
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| 作者姓名： | 吴燕萍 金其荣 |
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| 作者单位： | 无锡轻工大学生物工程学院!无锡214036 |
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| 摘 要： | 对产普鲁兰酶的出发菌株进行筛选和诱变，使酶活从22．10u／ml提高到了40．77u／ml，酶活提高了84．4％。随后对菌体产酶培养基进行了确定和优化，得到最佳发酵培养基，在此培养基下，菌体产酶达46．76u／ml，为原酶活的114．7％。
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| 关 键 词： | 普鲁兰酶 诱变 发酵 优化 Bacillussp WX5 |
STUDY ON PRODUCTION OF PULLULANASE BY MICROORGANISM |
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| Wu Yanping, Jin Qirong, Li Xun.STUDY ON PRODUCTION OF PULLULANASE BY MICROORGANISM[J].Biotechnology,1998,8(6):14-17. |
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| Authors: | Wu Yanping Jin Qirong Li Xun |
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| Abstract: | A pullulanase-producing strain was screened and mutated with DES by which the pul lulanase activity was increased from 22. 10u/ml to 40. 77u/ml. The fermentation conditions and medium were studied and optimized. With the optimization fermentation medium, the enzyme activity reached 46. 76u/ml |
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| Keywords: | pullulanase mutation fermentation optimization Bacillus sp WX5 |
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