| 高效烟草遗传转化体系的建立及甜蛋白基因的导入 |
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| 引用本文： | 杨成丽,刘树楠,周吉源,刘德立.高效烟草遗传转化体系的建立及甜蛋白基因的导入[J].生物技术,2004,14(2):9-11. |
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| 作者姓名： | 杨成丽 刘树楠 周吉源 刘德立 |
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| 作者单位： | 1. 南京理工大学化工学院生物工程系,江苏,南京,210094
2. 华中师范大学生命科学院,湖北,武汉,430097 |
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| 摘 要： | 以烟草无菌茁叶片为外植体，通过根癌农杆菌LBA4404介导法，将Thamnatin基因导人烟草中，经梯度卡那霉素(Kana-mycin，Km)筛选，获得可在含75mg／L、100mg／L Km选择生根培养基上再生的抗性植株，其中部分Km抗性植株经PCR检测为阳性，转化率为31．3％，初步鉴定已成功地建立了烟草遗传转化系统，为进一步探讨甜蛋白在植物中的转化和表达情况奠定基础。
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| 关 键 词： | 烟草 遗传转化 甜蛋白基因 根癌农杆菌 转基因 |
| 文章编号： | 1004-311X(2004)02-0009-03 |
Introduction of Thaumatin Gene into Tobacco(Nicotiana tabacum L.)through Agrobacterium-mediated Genetic Transformation |
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| YANG Cheng-li,LIU Shu-nan,ZHOU Ji-yuan,LIU De-li.Introduction of Thaumatin Gene into Tobacco(Nicotiana tabacum L.)through Agrobacterium-mediated Genetic Transformation[J].Biotechnology,2004,14(2):9-11. |
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| Authors: | YANG Cheng-li LIU Shu-nan ZHOU Ji-yuan LIU De-li |
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| Institution: | YANG Cheng-li~1,LIU Shu-nan~2,ZHOU Ji-yuan~2,LIU De-li~ |
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| Abstract: | The explant of leaf from tobacco sterile plants were transformed by Agrobaterium tumefaciens LBA4404.And through Agrobacterium-mediated system,the transformants were obtained by transferring explants to selection medium containing 75mg/L kanamycin and rooting medium containing 75mg/L,100mg/L kanamycin.The PCR assay of kan-resistant plants showed that the target gene had been integrated into tobacco accompany with NptII gene.The frequency of transformation was 31.3%. |
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| Keywords: | Thaumatin gene Agrobacterium tumfaciens Transgenic Nicotiana tabacum L Transformation |
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