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SRAP分子标记分析西瓜遗传多态性
引用本文:李晓慧,田朝阳,王从彦. SRAP分子标记分析西瓜遗传多态性[J]. 生物技术, 2007, 17(3): 23-26
作者姓名:李晓慧  田朝阳  王从彦
作者单位:1. 河南省农业科学院园艺所,河南,郑州,450002
2. 河南农业大学生命科学学院,河南,郑州,450002
摘    要:目的:探讨西瓜遗传多样性和遗传基础。方法:采用SRAP分子标记对西瓜品种D1、D2、D3、H1、H2、H3、M1、M2、M3、m1、m2、m3的多态性进行了分析。结果:每对引物组合产生13~25对比较清晰的扩增带.8对引物组合共产生131条扩增带。平均每对引物组合产生16.375条。8对引物组合共产生多态性带37条,每对引物组合产生3~7条,平均4.625条。每对引物组合产生的多态性带的比例为16.666%~38.464%,平均为28.675%。另外,对银染过程进行了优化。结论:SRAP标记多态性还是较高的,可以适于分析西瓜等遗传差异小的作物。

关 键 词:西瓜  SRAP分子标记  遗传多态性
文章编号:1004-311X(2007)03-0023-04
收稿时间:2007-01-07
修稿时间:2007-01-072007-03-03

Genetic Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Citrullus lanatus
LI Xiao-hui,TIAN Chao-yang,WANG Cong-yan. Genetic Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Citrullus lanatus[J]. Biotechnology, 2007, 17(3): 23-26
Authors:LI Xiao-hui  TIAN Chao-yang  WANG Cong-yan
Affiliation:1. Institute of Horficuhure, Henan Academy of Agricuhure sciences, Zhengzhou 450002, China; 2. College of Life Sciences, Henan Agricultural University , Zhengzhou 450002, China
Abstract:Objective:In order to investigate the Genetic diversity and genetic basis of Citrullus lanatus.Methods:Polymorphism among D1,D2,D3,H1,H2,H3,M1,M2,M3,m1,m2,m3 were analyzed with SRAP markers.Results:There were 13-25 clear combination bands per primer.About 131 amplified fragments and 37 polymorphic fragments were detected 8 SRAP primer combinations.There were 3-7 clear polymorphic combination bands per primer. The number of amplified fragments and polymorphic fragments per primer combination were 16.375 and 4.625 respectively.The percentage of polymorphic fragments was 16.666%-38.464%,average 28.675%. In addtion,optimizated the reaction of silver dyeing.Conclusion:All these results showed that the Polymorphism of SRAP markers is highely,and could be widely used in analyzed crops such as citrullus lanatus which has small Genetic Difference.
Keywords:watermelon  SRAP molecular marking  inherit diversity
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