| 高原鼠兔低氧诱导因子-1α的初步研究 |
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| 引用本文： | 赵同标,赵新全,常智杰,朱姗姗,赵伟,孙平,徐世晓.高原鼠兔低氧诱导因子-1α的初步研究[J].兽类学报,2003,23(3):273-276. |
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| 作者姓名： | 赵同标 赵新全 常智杰 朱姗姗 赵伟 孙平 徐世晓 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所,西宁,810001
2. 清华大学生物科学与技术系基因组研究所,北京,100084 |
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| 基金项目： | 国家基础研究规划项目 (G19980 40 813 )，中国科学知识创新工程项目 (KZCX1 0 1 0 1)，中国科学院海北高寒草甸生态系统开放试验站基金 |
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| 摘 要： | Hypoxia-induced factor-1 plays a key role during the cell hypoxia trausduction. Hypoxia induced factor-1α (HIF-1α) is a functional subunit of hypoxia-indueed factor-1. Plateau pika ( Ochotona curron/ae), which is a Qinghai-Tibet plateau native animal lived above 3 000 m, has high ratio of oxygen utilization to adapt to plateau hypoxia environments. One fragment of the coding re-gion of eDNA sequence of plateau pika HIF-1α was obtained by RT-PCR technique using a degeneracy PCR primer based on previ-ously reported eDNA sequence in human, cattle, house mouse and Norway rat HIF-1α gene. It was directly inserted into the vector pMD18-T. DNA sequencing proved that it was highly homology with human (91%), cattle (91%), house mouse (89%) and Nor-way rat 89%) HIF-1α gene. The study provides basic important information for the HIF-1α cDNA whole sequence cloning of pla-teau pika and its functional study.
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| 关 键 词： | 高原鼠兔 低氧诱导因子—1α 红细胞生成素 增强子 |
| 文章编号： | 1000-1050(2003)03-0273-04 |
| 修稿时间： | 2002年9月17日 |
A Preliminary Study of Plateau Pika (Ochotona curzoniae) Hypoxia-Induced Factor-1α |
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| ZHAO Tongbiao,ZHAO Xinquan,CHANG Zhijie,ZHU Shanshan,ZHAO W ei,SUN Ping,XU Shixiao.A Preliminary Study of Plateau Pika (Ochotona curzoniae) Hypoxia-Induced Factor-1α[J].Acta Theriologica Sinica,2003,23(3):273-276. |
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| Authors: | ZHAO Tongbiao ZHAO Xinquan CHANG Zhijie ZHU Shanshan ZHAO W ei SUN Ping XU Shixiao |
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| Institution: | ZHAO Tongbiao 1 ZHAO Xinquan 1 CHANG Zhijie 2 ZHU Shanshan 2 ZHAO W ei 1 SUN Ping 1 XU Shixiao 1 |
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| Abstract: | |
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| Keywords: | RT-PCR |
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