| 黑麂MHC-DQA2基因多态性 |
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| 引用本文： | 朱飞虎,闫计春,骆涛林,虞健,吴海龙.黑麂MHC-DQA2基因多态性[J].兽类学报,2011,31(1):90-96. |
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| 作者姓名： | 朱飞虎 闫计春 骆涛林 虞健 吴海龙 |
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| 作者单位： | 安徽师范大学生命科学学院,安徽省高校生物环境与生态安全省级重点实验室,安徽省重要生物资源保护与利用省级重点实验室,芜湖,241000 |
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| 基金项目： | 国家自然科学基金资助项目，安徽省优秀青年基金资助项目，安徽省自然科学基金资助项目 |
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| 摘 要： | 利用牛特异性扩增DQA2第2外显子的嵌套引物,对黑麂基因组DNA进行PCR扩增和克隆测序,基于该序列设计出黑麂DQA2基因第2外显子特异性引物.利用该引物,通过PCR-SSCP以及克隆测序技术,从40个黑麂样品中获得4个不同的DQA2等位基因.没有一个个体同时具有2个以上的等位基因,所有序列均不含插入或缺失突变,不含终...
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| 关 键 词： | 黑麂 MHC DQA2 多态性 平衡选择 跨物种进化 |
Polymorphism of MHC-DQA2 in black muntjac(Muntiacus crinifrons) |
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| ZHU Feihu,YAN Jichun,LUO Taolin,YU Jian,WU Hailong.Polymorphism of MHC-DQA2 in black muntjac(Muntiacus crinifrons)[J].Acta Theriologica Sinica,2011,31(1):90-96. |
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| Authors: | ZHU Feihu YAN Jichun LUO Taolin YU Jian WU Hailong |
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| Institution: | ZHU Feihu,YAN Jichun,LUO Taolin,YU Jian,WU Hailong(Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province,Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources,College of Life Science,Anhui Normal University,Wuhu 241000,China) |
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| Abstract: | A set of nested primers specific to cattle DQA loci was used to amplify genomic DNA of the black muntjac(Muntiacus crinifrons)using PCR.The obtained sequences showed high similarity to those of DQA2 exon 2 sequences from cattle,sheep,and sika deer.Based on the sequences,a new pair of primers specific to DQA2 locus of the black muntjac was designed and used to analyze samples of this species using PCR-SSCP and sequencing.In total,four distinct DQA2 exon 2 sequences were obtained from forty individuals and no... |
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| Keywords: | MHC DQA2 |
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