黑线姬鼠两亚种(中国东北地区及俄罗斯远东地区的东北亚种和朝鲜半岛的朝鲜亚种)的线粒体DNA序列缺少趋异性

为了检测黑线姬鼠两亚种(来自中国东北地区、俄罗斯远东地区的东北亚种和朝鲜半岛的朝鲜亚种)线粒体DNA的变异水平并确定朝鲜亚种的分类地位,我们测序分析了两亚种的线粒体DNA细胞色素b的部分序列(1 054 bp)和控制区的部分序列(860 bp),并与基因库中黑线姬鼠相应的单倍型序列进行了比较.可以看出东北亚种的序列显示...

Lack of mitochondrial DNA sequence divergence in two subspecies of the striped field mouse(Apodemus agrarius mantchuricus from Northeastern China and nearby Far-eastern Russia and A.a.coreae from the Korean Peninsula)

To determine the degree of mitochondrial DNA(mtDNA) divergence between two subspecies of Apodemus agrarius(A.a.mantchuricus from Northeastern China and nearby far-eastern Russia and A.a.coreae from the Korean peninsula) and to confirm the taxonomic status of the Korean subspecies,we obtained partial sequences of the mtDNA cytochrome b gene(1 054 bp) and the control region(860 bp) of these two subspecies.These sequences were compared to the corresponding haplotypes of these two subspecies,obtained from GenBank.It was observed that the A.a.mantchuricus sequences were somewhat divergent and could be separated into two or three subgroups,and we propose further DNA analyses with additional specimens to confirm the subspecific status of A.a.mantchuricus. Additionally,the sequences of A.a.coreae,from Korea,were similar to the two subgroups in A.a.mantchuricus(one subgroup of two haplotypes in the cytochrome b; another subgroup of two haplotypes in the control region),from Longjiang and Harbin in Northeastern China,indicating that the genetic diversity of these mice based on mtDNA sequencing is not concordant with their current classification based on morphological characters.We conclude that A.a.coreae from Korea is an endemic subspecies,with only morphological distinctness from A.a.mantchuricus,though we propose further analyses with other DNA markers to reexamine the subspecies status of A.a.coreae. We also conclude that the Korean peninsula did not act as a refugium for A.agrarius during the last glaciation.