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| 海南坡鹿CDC42 cDNA的克隆、原核表达及纯化 | |
| 引用本文： | 张巍,陈品林,雷明,成鹰,陈欢,曹献英,杜丽,张冬琳,刘涛,许世英,符运南,祁超,王凤阳.海南坡鹿CDC42 cDNA的克隆、原核表达及纯化[J].兽类学报,2011,31(1):97-102. |
| 作者姓名： | 张巍 陈品林 雷明 成鹰 陈欢 曹献英 杜丽 张冬琳 刘涛 许世英 符运南 祁超 王凤阳 |
| 作者单位： | 1. 海南大学农学院,海口市动物基因工程重点实验室,海口,570228;华中师范大学生命科学学院,武汉,430079 2. 海南大学农学院,海口市动物基因工程重点实验室,海口,570228 3. 海南大田国家级自然保护区,东方,572600 4. 华中师范大学生命科学学院,武汉,430079 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 应用RT-PCR方法对海南坡鹿细胞分裂周期蛋白42(Cell division cycle 42,CDC42)编码区进行扩增,将扩增产物与PET-42a载体连接,重组质粒鉴定正确后,进行生物信息学分析;构建pET42a-hdCDc42表达载体,经IPTG诱导表达,将表达产物进行SDS-PAGE、可溶性分析、纯化及Wes... |
| 关 键 词： | 海南坡鹿 CDC42 克隆 原核表达 纯化 |
Cloning and prokaryotic expression and purification of CDC42 cDNA from Hainan Eld's Deer |
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| ZHANG Wei,CHEN Pinlin,LEI Ming,CHENG Ying,CHEN Huan,CAO Xianying,DU Li,ZHANG Donglin,LIU Tao,XU Shiying,FU Yunnan,QI Chao,WANG Fengyang.Cloning and prokaryotic expression and purification of CDC42 cDNA from Hainan Eld's Deer[J].Acta Theriologica Sinica,2011,31(1):97-102. | |
| Authors: | ZHANG Wei CHEN Pinlin LEI Ming CHENG Ying CHEN Huan CAO Xianying DU Li ZHANG Donglin LIU Tao XU Shiying FU Yunnan QI Chao WANG Fengyang |
| Institution: | ZHANG Wei1,2,CHEN Pinlin1,LEI Ming1,CHENG Ying1,CHEN Huan1,CAO Xianying1,DU Li1,ZHANG Donglin1,LIU Tao1,XU Shiying3,FU Yunnan3,QI Chao2,WANG Fengyang1(1 College of Agriculture,Hainan University,Animal Genetic Engineering Key Laboratory of Haikou,Haikou 570228,China)(2 College of Life Sciences,Huazhoung Normal University,Wuhan 430079,China)(3 Hainan Datian National Reserve,Dongfang 572600,China) |
| Abstract: | Cell division cycle 42(CDC42) cDNA of Hainan Eld's Deer(Cervus eldi hainanus)was amplified by RT-PCR,subcloned into a pET42a vector and transformed into E.coli host cells.Protein expression was induced by IPTG and analyzed by SDS-PAGE and Western blot.The results showed that the CDC42 cDNA from Hainan Eld's Deer(hdCDC42)contained a 576 bp ORF encoding 191 amino acids.A 54 kD fusion protein with a Histag was induced by IPTG and confirmed by Western blot using anti His-tag monoclonal antibody.The results indi... |
| Keywords: | CDC42 |
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