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| HS-SPME-GC/MS在大熊猫尿液化学成分检测中的应用 | |
| 引用本文： | 刘玉良,辛秋红,刘定震,蔡志刚,张志和,兰景超,魏荣平,李德生,侯蓉.HS-SPME-GC/MS在大熊猫尿液化学成分检测中的应用[J].兽类学报,2012,32(1):48-57. |
| 作者姓名： | 刘玉良 辛秋红 刘定震 蔡志刚 张志和 兰景超 魏荣平 李德生 侯蓉 |
| 作者单位： | 1. 成都大熊猫繁育研究基地,四川省濒危野生动物保护生物学重点实验室/省部共建国家重点实验室培育基地,成都610081 2. 北京师范大学生态学研究所,生物多样性与生态工程教育部重点实验室,北京100875 3. 卧龙中国保护大熊猫研究中心,四川,623006 |
| 基金项目： | 成都市建委-大熊猫性外源激素研究项目 |
| 摘 要： | 尿液在大熊猫化学通讯过程中具有重要作用.对大熊猫尿液中化学成分的检测是揭示大熊猫尿液中化学物质组成及其功能的关键.本实验通过使用顶空固相微萃取技术(Headspace-solid phase microextraction,HSSPME)对大熊猫尿液样品进行前期处理,继而利用气相色谱-质谱联用技术(Gas chromatography-mass spectrometry,GC/MS)对大熊猫尿液中化学成分进行检测.共检测到56个峰,通过在NIST(National Institute of Standardsand Technology)质谱库中进行检索,初步推定出其中的38种物质.除此之外,还对HS-SPME萃取头的净化方法进行了探索和改进.结果表明,顶空固相微萃取技术结合气相色谱-质谱联用技术能够应用于大熊猫尿液中挥发性与半挥发性化合物的检测,并且能够得到较好的实验结果,为揭示大熊猫化学通讯机理提供基础. |
| 关 键 词： | 大熊猫 尿液 顶空固相微萃取 气相色谱-质谱联用 化学物质组成 |
The application of HS-SPME-GC/MS in detecting chemical components in giant panda(Ailuropoda melanoleuca)urine |
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| LIU Yuliang,XIN Qiuhong,LIU Dingzhen,CAI Zhigang,ZHANG Zhihe,LAN Jingchao,WEI Rongping,LI Desheng,HOU Rong.The application of HS-SPME-GC/MS in detecting chemical components in giant panda(Ailuropoda melanoleuca)urine[J].Acta Theriologica Sinica,2012,32(1):48-57. | |
| Authors: | LIU Yuliang XIN Qiuhong LIU Dingzhen CAI Zhigang ZHANG Zhihe LAN Jingchao WEI Rongping LI Desheng HOU Rong |
| Institution: | 1(1 Chengdu Research Base of Giant Panda Breeding;The Key Laboratory for Conservation Biology of Endangered Wildlife,Sichuan Province /Developmental Status for State Key Laboratory in a Joint Venture Between the Ministry of Science and Technology of P.R.C and Sichuan Province,Chengdu 610081,China)(2 Ministry of Education,Key Laboratory of Biodiversity Science and Ecological Engineering,Institute of Ecology,Beijing Normal University,Beijing 100875,China)(3 China Conservation and Research Center for the Giant panda at Wolong,Sichuan 623006,China) |
| Abstract: | Urine plays an important role in chemical communication between giant pandas(Ailuropoda melanoleuca).The detection of chemical compounds in giant panda urine is the key to identifying the compositions and assaying the function of each chemical compound.We used headspace-solid phase microextraction(HS-SPME) and gas chromatography-mass spectrometry(GC/MS) to pre-treat and analyze the composition of giant panda urine.We found 56 peaks and through searching the mass spectrometry library of the National Institute of Standards and Technology(NIST),38 of them were putatively identified.In addition,we also modified the method of cleaning HS-SPME fiber.The results show that HS-SPME-GC/MS is an effective method to detect the volatile and semi-volatile compounds in giant panda urine.It is proved to be an important research tool for revealing the mechanism of chemical communication in the giant pandas. |
| Keywords: | Chemical composition Gas Chromatography-Mass Spectrometry(GC/MS) Giant Panda Headspace-Solid Phase Microextraction(HS-SPME) Urine |
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