大熊猫尸体组织LDH同工酶盘电泳观察

大熊猫是冰川时期残存至今的古老、稀有的珍贵动物,有“活化石”之称。对大熊猫的研究除野外调查(王朗自然保护区大熊猫调查组,1974)、形态解剖(张鹤宇等,1959;冯文和等,1984)、人工饲养繁殖(北京动物园,1974;冯文和等,1983;1984)等外,尚有生化技术和免疫学等研究方法(Sarich,1973;潘文石等,1982;罗昌容等,1984)。

THE DISC ELECTROPHORESIS OBSERVATIONS ON LDH ISOENZYME OF TISSUES OF GIANT PANDA CARCASS

This paper deals with disc electrophoresis of LDH isoenzyme of 16 tissues of giant panda carcasses, the 16 tissues are cerebrum, cerebellum, spinal cord, crystal, bone marrow, skeletal muscle, cardiac muscle, gastric liquid, liver, spleen, kidney, adrenal gland, lung, ovary, testis and epididymis. 5 giant panda carcasses have been observed.The 16 tissues of giant panda carcasses have been studied by polyacryl-amide gel disc electrophoresis and the results are as follows:1. 5 components of LDH isoenzyme band of 16 tissues of giant panda carcasses range, from positive electrode to negative electrode, LDH₁,LDH₂, LDH₃, LDH₄, LDH₅.2. The activity of LDH₁ is higher, while the activities of LDH₄ and LDH₆ are lower in cerebrum, cerebellum, spinal cord, cardiac muscle, kidney, adrenal gland and epididymis; the activity of LDH₅ is higher, while the activities of LDH₁ and LDH₂ are lower in gastric liquid, skeletal muscle and liver; the activity of LDH₂ is higher, while the activities of LDH₄ and LDH₅ are lower in crystal, bone marrow, ovary and testis; the activity of LDH₃ is higher, while the activity of LDH₅ is lower in spleen and lung.