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圈养小熊猫几种病毒的PCR检测
引用本文:秦琴,张陕宁,李明,魏辅文.圈养小熊猫几种病毒的PCR检测[J].兽类学报,2006,26(4):387-391.
作者姓名:秦琴  张陕宁  李明  魏辅文
作者单位:1. 中国科学院动物研究所动物生态与保护生物学重点实验室,北京,100080;中国科学院研究生院,北京,100049
2. 中国科学院动物研究所动物生态与保护生物学重点实验室,北京,100080;中国野生动物保护协会,北京,100080
3. 中国科学院动物研究所动物生态与保护生物学重点实验室,北京,100080
基金项目:中国科学院动物研究所动物疫病研究中心疾病专项,国家林业局野生动物疾病监测资助项目
摘    要:本文采用巢式PCR/RT-PCR方法,对我国10个动物园中无临床症状的圈养小熊猫的71个肛拭子和61个唾液拭子样品,进行犬瘟热病毒(CDV)、犬细小病毒(CPV)、犬冠状病毒(CCV)、犬腺病毒(CAV)和犬疱疹病毒(CHV)的检测,以评估我国圈养小熊猫是否面临这几种病毒的威胁。对阳性PCR结果进行测序分析,并与GenBank上的序列进行比较。结果,在肛拭子样品中检测到3个CPV和6个CCV阳性结果,经测序后,与GenBank上序列的同源性分别达99%和100%。而在唾液拭子样品中没有检测到任何阳性结果,且CDV、CAV和CCV的检测结果均为阴性。从阳性CPV的肛拭子样品中分离到一株细小病毒毒株,表明圈养小熊猫已受到细小病毒和犬冠状病毒的感染,今后应加强这两种病毒的预防工作。本文所采用的PCR方法检测病毒性疾病,能检测到微量的病毒模板,可对小熊猫病毒性感染进行早期诊断。

关 键 词:PCR  小熊猫  犬细小病毒  犬冠状病毒
文章编号:1000-1050(2006)04-0387-05
收稿时间:04 4 2006 12:00AM
修稿时间:06 17 2006 12:00AM

PCR detection of selected viruses in captive red pandas (Ailurus fulgens) in China
QIN Qin,ZHANG Shanning,LI Ming,WEI Fuwen.PCR detection of selected viruses in captive red pandas (Ailurus fulgens) in China[J].Acta Theriologica Sinica,2006,26(4):387-391.
Authors:QIN Qin  ZHANG Shanning  LI Ming  WEI Fuwen
Institution:1 Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, the Chinese Academy of Sciences, Beijing, 100080 China; 2 Graduate School of Chinese Academy of Sciences, Beijing, 100049, China;3 China Wildlife Conservation Society, Beijing, 100080, China
Abstract:We used nested PCR/RT-PCR method to detect canine distemper virus(CDV),canine parvovirus(CPV),canine adenovirus(CAV),canine coronavirus(CCV),and canine herpesvirus(CHV)in 71 rectal and 61 saliva swabs from captive red pandas without any clinical signs in ten zoos in China to evaluate the threat of captive red pandas by these viruses.Positive PCR products were sequenced and compared with those from the GenBank.We detected three CPV and six CCV positive results from rectal swabs samples,but we found nothing in saliva samples and didn't detect CDV,CAV and CHV in any sample.The homologies of CPV and CCV sequences between PCR positive samples and the GenBank are 99%-100%,respectively.One parvovirus strain was isolated from one of the three parvovirus positive samples.These results showed captive red pandas in China were infected with CPV and CCV,so we should place more emphasis on the prevention of CPV and CCV for better conservation of red pandas.The PCR method can detect trace template of virus and may be a diagnostic method for detecting early viral infection of red pandas.
Keywords:Canine parvovirus  Canine coronavirus  PCR  Red panda(Ailurus fulgens)
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