通过粪球形态快速鉴定马鹿性别的方法与分子鉴定

通过SRY 基因扩增,对76 份野生东北马鹿粪便DNA 进行了性别鉴定,其中雄性49 份,雌性27 份。观察发现粪球形态可分两类:子弹状、枣核状。子弹状呈短粗型,长宽比较小;枣核状呈细长型,长宽比较大。对76 份样品进行分类:子弹状51 份、枣核状25 份。并以样品长、宽平均值的比值(R)为指标快速聚类,并建立了判别方程。统计指出判别结果与实际性别吻合率79.71% ;形态分类与实际性别吻合率90.56% ,即子弹状为雄性,枣核状为雌性。结果提示今后的野外研究可直接利用粪球形态判定粪样性别,长宽比判别方程可作为辅助。

A molecular identification and rapid recognition approach for the sex determination of wild wapiti by feces pellet morphology

The DNA sex of wild wapiti was determined by PCR amplification of the SRY genes of 76 feces samples. Among
of them,49 samples were identified as male,and the others as female. All fecal pellets presented one of two shapes:bullet-like and jujube-stone like. The bullet-like fecal pellets displayed a short thick form with a low length/ width ratio. Thejujube-stone like ones displayed a thin long form with high length/ width ratio. Using these shapes,the 76 samples were divided into two groups:51 bullet-like and 25 jujube-stone like feces. All the samples were clustered by the ratio of averaged length and width,and then the discrimination equation was established. The statistical results showed that the consistent degree of sex determination by fecal statistic index and DNA method was 79. 71% ,while morphological method and real sex was 90. 56% . So,the bullet-like pellets are from male wapiti,and the jujube-stone like ones from females. Our results show that wapiti sex can be directly determined by its pellet shape in the wild. At the same time,the discrimination equation could be used as associated method.