| 盘羊肺炎支原体感染PCR检测方法的建立与初步应用 |
| |
| 引用本文： | 冷青文,李志远,屈勇刚,鲁海富,金云云,王静梅,剡根强.盘羊肺炎支原体感染PCR检测方法的建立与初步应用[J].兽类学报,2011,31(3):312-316. |
| |
| 作者姓名： | 冷青文 李志远 屈勇刚 鲁海富 金云云 王静梅 剡根强 |
| |
| 作者单位： | 石河子大学动物科技学院,石河子,832003 |
| |
| 基金项目： | 教育部博士学科点专项科研基金资助项目(20060759001) |
| |
| 摘 要： | 羊支原体性肺炎,是由支原体引起的一类高度接触性传染病,又称羊传染性胸膜肺炎(Infections pleuropneumonia of sheep and goats),临床症状主要表现为高热、咳嗽、消瘦、
|
| 关 键 词： | 盘羊 PCR 绵羊肺炎支原体 |
Establishment and application of PCR for diagnose Mycoplasma ovipneumoniae of Argali(Ovis ammon) |
| |
| LENG Qingwen,LI Zhiyuan,QU Yonggang,LU Haifu,JIN Yunyun,WANG Jingmei,YAN Genqiang.Establishment and application of PCR for diagnose Mycoplasma ovipneumoniae of Argali(Ovis ammon)[J].Acta Theriologica Sinica,2011,31(3):312-316. |
| |
| Authors: | LENG Qingwen LI Zhiyuan QU Yonggang LU Haifu JIN Yunyun WANG Jingmei YAN Genqiang |
| |
| Institution: | LENG Qingwen,LI Zhiyuan,QU Yonggang,LU Haifu,JIN Yunyun,WANG Jingmei,YAN Genqiang*(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China) |
| |
| Abstract: | To detect infection of Mycoplasma ovipneumoniae(MO) of Argali(Ovis ammon),we designed a pair of specific primers to amplify a 406 bp fragment of the 16S rRNA gene of MO.After analysis of the optimization reaction condition,e.g.,the specificity and sensitivity for PCR of the MO gene,anneal temperature,concentration of Taq DNA polymerase and that of the primers,the sensitivity of PCR was found to be 0.4075 ng genome DNA for MO.A 406 bp fragment was amplified from standard strain of MO Y98,and another was an i... |
| |
| Keywords: | Argali(Ovis ammon) Mycoplasma ovipneumoniae(MO) PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
