圈养大熊猫血清微量元素含量的测定与分析

正微量元素包括Fe、Cu、Cr、Mn和Zn等,虽含量极微,但具有极重要的生理功能,涉及机体的生长发育、免疫代谢等生命活动过程(Shenkin,2008; Subrahmanyam et al.,2015),也是反映机体新陈代谢及生理功能状况的重要指标(Carpene et al.,2017)。例如Fe元素参与机体氧的运输和贮存(Abbaspour et al.,2014),血清铁被认为是动物营养评估的生化指标(Ganapathy et al.,2011); Cu元素是多种氧化酶的重要组分,参与动物新陈代谢、生长发育等(Desai et al.,2014)。

Determination and analysis of trace elements in serum of captive giant pandas

The background values of trace elements are used to evaluate the levels in giant pandas, also provide a scientific basis for the prevention, diagnosis of diseases and rational feeding methods. To establish the background values and further improve the database of trace elements in serum of giant panda, this paper presents results from 97 blood samples collected under non-anesthesia condition by caring rearing and medical training of captive giant pandas from CCRCGP. The contents of Fe, Cu, Zn, Mn, Cr, Co, Ni, Pb and Cd in serum determined by atomic absorption spectrometry were 4830 μg/L, 1000 μg/L, 3220 μg/L, 38.37 μg/L, 4.79 μg/L, 23.49 μg/L, 4.76 μg/L, 3.02 μg/L and 1.47 μg/L, respectively. This paper analyzes the micronutrient levels in different sex and age groups by two-factor variance analysis. It is shown that the contents of essential Fe, Cu, Zn, Mn, Cr, Co, Ni were normal, stable, and no significant difference between sex and age. The contents of harmful Pb, Cd were similar in different gender, but there was an accumulation trend with age within the safe range. The trace elements nutritional status in captive giant pandas is comfortable, furthermore, the feeding management and habitat environment suit for giant panda protection.