Cytological and molecular characterization of a chromosome interchange and addition lines in Cadet involving chromosome 5B of wheat and 6Ag of Lophopyrum ponticum |
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| Authors: | N -S Kim K C Armstrong G Fedak A Fominaya E W P Whelan |
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| Institution: | (1) Plant Research Centre, Research Branch, Agriculture Canada, K1A OC6 Ottawa, Canada;(2) Department of Cell Biology and Genetics, University of Alcala de Henares, Alcala de Henares, 28871 Madrid, Spain;(3) Agriculture Canada Research Station, T1J 4B1 Lethbridge, Alberta, Canada |
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| Abstract: | Efforts to transfer wheat curl mite (Eriophyes tulipae Keifer) resistance from Lophopyrum ponticum 10X (Podb.) Love to bread wheat (Triticum aestivum L.) have resulted in the production of a number of cytogenetic stocks, including an addition line of 6Ag, a  ditelo addition line, and a wheat-Lophopyrum translocation line. Characterization of these lines with C-banding, in situ hybridization with a Lophopyrum species-specific repetitive DNA probe (pLeUCD2), and Southern blotting with pLeUCD2 and a 5S ribosomal DNA probe (pScT7) confirmed that the distal portion of the short arm of 6Ag was translocated onto the distal portion of 5BS (5BL. 5BS-6AgS). It was also determined that the ditelo addition was an acrocentric chromosome of 6AgS. |
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| Keywords: | Wheat Lophopyrum Translocation Repetitive DNA sequence C-banding In situ hybridization |
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