RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper ( Nilaparvata lugens) |
| |
Authors: | Xu X F Mei H W Luo L J Cheng X N Li Z K |
| |
Institution: | (1) Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, DAPO 7777, Metro Manila, The Philippines e-mail: z.li@cgiar.org, PH;(2) Nanjing Agricultural University, Nanjing, China, CN;(3) China National Rice Research Institute, Hangzhou, China, CN |
| |
Abstract: | Quantitative trait loci (QTLs), conferring quantitative resistance to rice brown planthopper (BPH), were investigated using
160 F11 recombinant inbred lines (RILs) from the Lemont/Teqing cross, a complete RFLP map, and replicated phenotyping of seedbox
inoculation. The paternal indica parent, Teqing, was more-resistant to BPH than the maternal japonica parent, Lemont. The
RILs showed transgressive segregation for resistance to BPH. Seven main-effect QTLs and many epistatic QTL pairs were identified
and mapped on the 12 rice chromosomes. Collectively, the main-effect and epistatic QTLs accounted for over 70% of the total
variation in damage scores. Teqing has the resistance allele at four main-effect QTLs, and the Lemont allele resulted in resistance
at the other three. Of the main-effect QTLs identified, QBphr5b was mapped to the vicinity of gl1, a major gene controlling leaf and stem pubescence. The Teqing allele controlling leaf and stem pubescence was associated
with resistance, while the Lemont allele for glabrous stem and leaves was associated with susceptibility, indicating that
this gene may have contributed to resistance through antixenosis. Similar to the reported BPH resistance genes, the other
six detected main-effect QTLs were all mapped to regions where major disease resistance genes locate, suggesting they might
have contributed either to antibiosis or tolerance. Our results indicated that marker-aided pyramiding of major resistance
genes and QTLs should provide effective and stable control over this devastating pest.
Received: 10 December 2000 / Accepted: 7 May 2001 |
| |
Keywords: | Insect resistance QTL mapping Antiexenosis Epistasis |
本文献已被 PubMed SpringerLink 等数据库收录! |
|