SSR mapping and confirmation of the QTL from PI96354 conditioning soybean resistance to southern root-knot nematode |
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Authors: | Z Li L Jakkula R S Hussey J P Tamulonis H R Boerma |
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Institution: | (1) Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602, USA e-mail: rboerma@arches.uga.edu Fax: +1-(706) 542-0560, US;(2) Department of Plant Pathology, University of Georgia, Athens, GA 30602, USA, US;(3) Monsanto Company, 634 E. Lincoln Way, Ames, IA 50010, USA, US |
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Abstract: | Root-knot nematodes (Meloidogyne spp.) can cause severe yield loss of soybean Glycine max (L.) Merr.] in the southern production region of the USA. Planting root-knot nematode-resistant cultivars is the most effective
method of preventing yield loss. DNA marker-assisted breeding may accelerate the development of root-knot nematode-resistant
cultivars. RFLP markers have previously been used to identify quantitative trait loci (QTLs) conferring resistance to southern
root-knot nematode Meloidogyne incognita (Kofoid and White) Chitwood] (Mi) in a F2:3 soybean population created by crossing the resistant PI96354 and the susceptible ’Bossier.’ A major QTL on linkage group
(LG) O conditioning 31% of the variation in Mi gall number and a minor QTL on LG-G conditioning 14% of the gall variation
were reported. With the development of SSR markers for soybean improvement, a higher level of mapping resolution and semi-automated
detection has become possible. The objectives of this research were: (1) to increase the marker density in the genomic regions
of the QTLs for Mi resistance on LG-O and LG-G with SSR markers; and (2) to confirm the effect of the QTLs in a second population
and a different genetic background. With SSR markers, the QTL on LG-O was flanked by Satt492 and Satt358, and on LG-G by Satt012
and Satt505. Utilizing SSR markers flanking the two QTLs, marker-assisted selection was performed in a second F2:3 population of PI96354× Bossier. Results confirmed the effectiveness of marker-assisted selection to predict the Mi phenotypes.
By screening the BC2F2 population of Prichard (3)×G93–9009 we confirmed that selection for the minor QTL on LG-G with flanking SSR markers would
enhance the resistance of lines containing the major QTL (which is most-likely Rmi1).
Received: 29 September 2000 / Accepted: 17 April 2001 |
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Keywords: | Marker-assisted selection Quantitative trait loci Simple sequence repeats Meloidogyne incognita Glycine max |
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