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Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust
Authors:B Stirling  G Newcombe  J Vrebalov  I Bosdet  HD Bradshaw Jr
Institution:(1) Center for Urban Horticulture, Box 354115, 3501 NE 41st St., University of Washington, Seattle, WA 98195-4115, USA e-mail: toby@u.washington.edu Tel.: +206 616-1796, Fax: +206 685-2692, US;(2) Department of Forest Resources, University of Idaho, Moscow, Idaho 83844-1183, USA, US;(3) Boyce Thompson Institute of Plant Research, Tower Road, Cornell University Campus, Ithaca, NY 14853, USA, US;(4) Genome Sequence Centre, B.C. Cancer Research Centre, Vancouver V5Z-4E6, B.C., Canada, CA
Abstract:A positional cloning strategy is being implemented in Populus for the isolation of the dominant MXC3 allele, which confers resistance to poplar leaf rust caused by Melampsora×columbiana (pathotype 3). AFLP markers were used to saturate the chromosomal region around the MXC3 locus in a large (n=1,902) Populus trichocarpa×P. deltoides (T×D) mapping pedigree segregating 1:1 for rust resistance and susceptibility. The high-resolution linkage map developed around the MXC3 locus contains 19 AFLP markers and spans a genetic distance of 2.73 cM. Of the 19 AFLP markers, seven were found to co-segregate with the locus. One co-segregating AFLP marker, CCG.GCT_01, was converted to an STS marker (BVS1) and used to identify a physical contig of overlapping BAC clones from the MXC3 region. Genetic and physical mapping of markers isolated from the BAC contig failed to delimit the MXC3 locus within a 300-kb interval defined by the overlapping BAC clones. This result indicates a >25-fold reduction in recombination frequency in the MXC3 region compared to the average rate of recombination for the Populus genome. Received: 8 December 2000 / Accepted: 1 March 2001
Keywords:  Map-based cloning  Hybrid poplar  Plant disease resistance
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