Inheritance and expression of the cry1Ab gene in Bt ( Bacillus thuringiensis) transgenic rice |
| |
| Authors: | Wu G Cui H Ye G Xia Y Sardana R Cheng X Li Y Altosaar I Shu Q |
| |
| Institution: | (1) Institute of Nuclear Agricultural Sciences, Zhejiang University, Hua Jia Chi, Hangzhou 310029, China e-mail: qyshu@zju.edu.cn Fax: +86-571-86971202, CN;(2) Institute of Applied Entomology, Zhejiang University, Hua Jia Chi, Hangzhou 310029, China, CN;(3) Agricultural Biotechnology Laboratories, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada e-mail: altosaar@vottawa.cd, CA;(4) Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China, CN |
| |
| Abstract: | The inheritance and expression patterns of the cry1Ab gene were studied in the progenies derived from different Bt (Bacillus thuringiensis) transgenic japonica rice lines under field conditions. Both Mendelian and distorted segregation ratios were observed in some selfed and crossed
F2 populations. Crosses between japonica intra-subspecies had no significant effect on the segregation ratios of the cry1Ab gene, but crossing between japonica and indica inter-subspecies led to distorted segregation of the cry1Ab gene in the F2 population. Field-release experiments indicated that the cry1Ab gene was stably transmitted in an intact manner via successive sexual generations, and the concentration of the Cry1Ab protein
was kept quantitatively stable up to the R6 generation. The cry1Ab gene, driven by the maize ubiquitin promoter, displayed certain kinds of spatial and temporal expression patterns under field conditions. The content of the Cry1Ab
protein varied in different tissues of the main stems, the primary tillers and the secondary tillers. Higher levels of the
Cry1Ab protein were found in the stems, leaves and leaf sheaths than in the roots, while the lowest level was detected in
grains at the maturation stage. The content of the Cry1Ab protein in the leaves peaked at the booting stage and was lowest
at the heading stage. Furthermore, the Cry1Ab content of cry1Ab expression in different tissues of transgenic rice varied individually with temperature.
Received: 17 April 2001 / Accepted: 7 May 2001 |
| |
| Keywords: | Inheritance Expression cry1Ab gene Bt (Bacillus thuringiensis) transgenic rice |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
