A chalcone synthase/stilbene synthase DNA probe for conifers |
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Authors: | S M Baker E E White |
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Institution: | (1) Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, V8Z 1M5 Victoria, B.C., Canada |
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Abstract: | A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes in Abies procera, Pinus lambertiana, P. monticola, Picea glauca, P. sitchensis, Pseudostuga menziesii, Taxus brevifolia, and Thuja plicata. A large number of hybridizing bands were found in all species except T. plicata which did not cross hybridize. The hybridization patterns are highly polymorphic between the species and are also polymorphic within several of them. |
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Keywords: | Pinus monticola D Don Chalcone synthase Stilbene synthase DNA probes Conifers |
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