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Development of an RFLP map in diploid alfalfa
Authors:E Charles Brummer  Joseph H Bouton  Gary Kochert
Institution:(1) Department of Agronomy, University of Georgia, 30602 Athens, GA, USA;(2) Department of Botany, University of Georgia, 30602 Athens, GA, USA;(3) Present address: Department of Agronomy, Iowa State University, 50011 Ames, IA, USA
Abstract:Summary We have developed a restriction fragment length polymorphism (RFLP) linkage map in diploid alfalfa (Medicago sativa L.) to be used as a tool in alfalfa improvement programs. An F2 mapping population of 86 individuals was produced from a cross between a plant of the W2xiso population (M. sativa ssp. sativa) and a plant from USDA PI440501 (M. sativa ssp. coerulea). The current map contains 108 cDNA markers covering 467.5 centimorgans. The short length of the map is probably due to low recombination in this cross. Marker order may be maintained in other populations even though the distance between clones may change. About 50% of the mapped loci showed segregation distortion, mostly toward excess heterozygotes. This is circumstantial evidence supporting the maximum heterozygote theory which states that relative vigor is dependent on maximizing the number of loci with multiple alleles. The application of the map to tetraploid populations is discussed.
Keywords:RFLP  Alfalfa  Genetic map  Segregation distortion  Plant breeding
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