Characterization of β-amylase and its deficiency in various rice cultivars |
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Authors: | J Yamaguchi S Itoh T Saitoh A Ikeda T Tashiro Y Nagato |
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Institution: | (1) Bioscience Centre, Nagoya University, Chikusa, Nagoya 464-8601, Japan Fax: +81-52-789-5226 E-mail: b42295a@nucc.cc.nagoya-u.ac.jp, JP;(2) Department of Agriculture, Meijo University, Tenpaku, Nagoya 468-0073, Japan, JP;(3) Experimental Farm, Faculty of Bioresources, Mie University, 2072-2 Takanoo-cho, Tsu-shi 514-2221, Japan, JP;(4) Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan, JP |
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Abstract: | β-Amylase deficiency in various cultivars of rice was examined at the molecular level. Using an antibody against β-amylase
purified from germinating seeds of rice, we were able to demonstrate the expression and organization of the β-amylase gene
in normal and deficient cultivars. Although β-amylase is a starch-hydrolyzing enzyme, as is α-amylase, the β-amylase protein/gene
is expressed differently from the α-amylase protein/gene; i.e. (1) β-amylase is synthesized only in aleurone cells, (2) the
enzyme production in the embryo-less half-seeds is not under hormonal control. We identified some cultivars of rice that are
deficient for β-amylase activity. We present new evidence that synthesis is blocked at the level of mRNA synthesis in the
deficient cultivars. The usefulness of β-amylase as a crop trait is also discussed.
Received: 8 May 1998 / Accepted: 5 June 1998 |
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Keywords: | β -amylase-deficient mutant Oryza sativa Germination Direct seedling |
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