Genetic mapping of hypervariable minisatellite sequences in rice (Oryza sativa L.) |
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| Authors: | J P Gustafson M Yano |
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| Institution: | (1) United States Department of Agriculture, Agricultural Research Service, Plant Genetics Research Unit, and Plant Science, Unit, University of Missouri, Columbia, Missouri 65211 USA, US;(2) Rice Genome Project (RGP), National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305, Japan, JP |
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| Abstract: | Minisatellites, or DNA fingerprinting sequences, have been utilized in animal linkage studies for several years but have not
been used as markers for plant genome mapping. In animal genome mapping they have resulted in limited success because they
are evenly dispersed in some species but are often clustered near telomeric regions, as observed on human chromosomes. The
purpose of the present study was to generate DNA fingerprints utilizing several rice-derived minisatellites containing different
core sequences and numbers of repeat units, followed by assessing their potential for use as genetic markers when mapped to
a rice recombinant inbred line (RIL) population. Sites of segregating minisatellite loci were mapped onto 11 of the 12 rice
RIL linkage maps. The implications for the use of rice minisatellite core sequences as genetic markers on linkage maps in
rice are discussed.
Received: 1 March 1999 / Accepted: 22 June 1999 |
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| Keywords: | Linkage maps Minisatellites DNA fingerprinting Rice |
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