Chromosome 5H of <Emphasis Type="Italic">Hordeum</Emphasis> species involved in reduction in grain hardness in wheat genetic background |
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Authors: | Mikiko Yanaka Kanenori Takata Yohei Terasawa Tatsuya M Ikeda |
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Institution: | (1) NARO Western Region Agricultural Research Center, 6-12-1, Nishifukatsu, Fukuyama Hiroshima, 721-8514, Japan; |
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Abstract: | Grain hardness is an important factor affecting end-use quality in wheat. Mutations of the puroindoline genes, which are located
on chromosome 5DS, control a majority of grain texture variations. Hordoindoline genes, which are the puroindoline gene homologs
in barley, are located on chromosome 5HS and are also responsible for grain texture variation. In this study, we used three
types of wheat–barley species (Hordeum vulgare, H. vulgare ssp. spontaneum, and H. chilense) chromosome addition lines and studied the effect of chromosome 5H of these species on wheat grain characteristics. The 5H
chromosome addition lines showed significantly lower grain hardness and higher grain weight than the corresponding wheat parents.
The effect of enhancing grain softness was largest in the wheat–H. chilense line regardless of having an increase in grain weight similar to those in the wheat–H. vulgare and wheat–H. spontaneum lines. Our results indicated that chromosome 5H of the Hordeum species plays a role in enhancing grain softness and increasing grain weight in the wheat genetic background, and the extent
of effect on grain hardness depends on the type of Hordeum species. Protein analysis of hordoindolines indicated that profiles of 2D-electrophoresis of hordoindolines were different
among Hordeum species and hordoindolines in the addition lines appeared to be most abundant in wheat–H. chilense line. The differences in enhancing grain softness among the Hordeum species might be attributed to the quantity of hordoindolines expressed in the 5H chromosome addition lines. These results
suggested that the barley hordoindolines located on chromosome 5HS play a role in reducing grain hardness in the wheat genetic
background. |
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