Molecular analysis of crosses between Tripsacum dactyloides and Zea diploperennis (Poaceae) |
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Authors: | M W Eubanks |
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Institution: | (1) Department of Botany, Box 90338, Duke University, Durham, NC 27708, USA, US |
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Abstract: | DNA fingerprinting verified hybrid plants obtained by crossing Eastern gamagrass, Tripsacum dactyloides L., and perennial teosinte, Zea diploperennis Iltis, Doebley & R. Guzmán. Pistillate inflorescences on these hybrids exhibit characteristics intermediate to the key morphological
traits that differentiate domesticated maize from its wild relatives: (1) a pair of female spikelets in each cupule; (2) exposed
kernels not completely covered by the cupule and outer glumes; (3) a rigid, non-shattering rachis; (4) a polystichous ear.
RFLP analysis was employed to investigate the possibility that traits of domesticated maize were derived from hybridization
between perennial teosinte and Tripsacum. Southern blots of restriction digested genomic DNA of parent plants, F1, and F2 progeny from two different crosses were probed with RFLP markers specifically associated with changes in pistillate inflorescence
architecture that signal maize domestication. Pairwise analysis of restriction patterns showed traits considered missing links
in the origin of maize correlate with alleles derived from Tripsacum, and the same alleles are stably inherited in second generation progeny from crosses between Tripsacum and perennial teosinte.
Received: 11 October 1996/Accepted:8 November 1996 |
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Keywords: | Maize Teosinte Zea Tripsacum RFLP analysis |
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