Positional cloning of <Emphasis Type="Italic">ds1</Emphasis>, the target leaf spot resistance gene against <Emphasis Type="Italic">Bipolaris sorghicola</Emphasis> in sorghum |
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Authors: | Hiroyuki Kawahigashi Shigemitsu Kasuga Tsuyu Ando Hiroyuki Kanamori Jianzhong Wu Jun-ichi Yonemaru Takashi Sazuka Takashi Matsumoto |
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Institution: | (1) National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan;(2) Faculty of Agriculture, Shinshu University, 8304, Minamiminowa, Nagano 399-4598, Japan;(3) Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba 305-0854, Japan;(4) Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya Aichi, 464-8601, Japan;(5) Plant Genome Research Unit, National Institute of Agrobiological Sciences, 2-1-2, Kan-non-dai, Tsukuba 305-8602, Japan |
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Abstract: | Target leaf spot is one of the major sorghum diseases in southern Japan and caused by a necrotrophic fungus, Bipolaris sorghicola. Sorghum resistance to target leaf spot is controlled by a single recessive gene (ds1). A high-density genetic map of the ds1 locus was constructed with simple sequence repeat markers using progeny from crosses between a sensitive variety, bmr-6,
and a resistant one, SIL-05, which allowed the ds1 gene to be genetically located within a 26-kb region on the short arm of sorghum chromosome 5. The sorghum genome annotation
database for BTx623, for which the whole genome sequence was recently published, indicated a candidate gene from the Leucine-Rich
Repeat Receptor Kinase family in this region. The candidate protein kinase gene was expressed in susceptible plants but was
not expressed or was severely reduced in resistant plants. The expression patterns of ds1 gene and the phenotype of target leaf spot resistance were clearly correlated. Genomic sequences of this region in parental
varieties showed a deletion in the promoter region of SIL-05 that could cause reduction of gene expression. We also found
two ds1 alleles for resistant phenotypes with a stop codon in the coding region. The results shown here strongly suggest that the
loss of function or suppression of the ds1 protein kinase gene leads to resistance to target leaf spot in sorghum. |
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