Plant regeneration in vitro from embryogenic cultures of spring- and winter-type barley (Hordeum vulgare L.) varieties |
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Authors: | R Lührs H Lörz |
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Institution: | (1) Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, Federal Republic of Germany |
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Abstract: | Summary Immature embryos of 41 lines of barley were screened in vitro for callus induction and somatic embryogenesis on different media to establish totipotent cultures. The use of modified MS and CC media, both supplemented with 1 g/l casein hydrolysate, and the substitution of agarose for agar resulted in the highest frequencies of somatic embryo induction. Embryogenic callus was induced and plants regenerated from 23 of the lines tested. The auxins 2,4-D, dicamba, picloram and 2,4,5-T were suitable for embryogenic callus induction. High frequencies of somatic embryo germination occurred on CC medium supplemented with 1 mg/l IAA and 0.05 mg/l zeatin. A strong genotypic effect on the capacity and frequency of embryogenic callus formation was found. Cultivar Golden Promise always gave the best results. Experiments with field grown material in 3 consecutive years showed that environmental factors also strongly influenced the induction of somatic embryogenesis and plant regeneration.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- 2,4,5-T
2,4,5-trichlorophenoxyacetic acid
- dicamba
3,6-dichloro-o-anisic acid
- picloram
4-amino-3,6,6-trichloropicolinic acid
- NAA
naphtaleneacetic acid
- IAA
indole-3-acetic acid
- ABA
abscisic acid
- BAP
6-benzyl amino purine
- 2iP
6-(3-methyl-2 butenyl 1-amino)purine
- GA3
gibberellic acid |
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Keywords: | Barley (Hordeum vulgare L ) Immature embryos Somatic embryogenesis Plant regeneration Genotypes |
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