Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis |
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Authors: | X M Chen R F Line H Leung |
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Institution: | (1) US Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164-6430, USA Fax: +1509-335-9581 E-mail: xianming@mail.wsu.edu, US |
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Abstract: | Genes cloned from diverse plants for resistance to different pathogens have sequence similarities in domains presumably involved
in pathogen recognition and signal transduction in triggering the defense response. Primers based on the conserved regions
of resistance genes often amplify multiple fragments that may not be separable in an agarose gel. We used denaturing polyacrylamide-gel
electrophoresis to detect PCR products of plant genomic DNA amplified with primers based on conserved regions of resistance
genes. Depending upon the primer pairs used, 30–130 bands were detected in wheat, rice, and barley. As high as 47%, 40%, and
27% of the polymorphic bands were detected in rice, barley, and wheat, respectively, and as high as 12.5% of the polymorphic
bands were detected by certain primers in progeny from a cross of the wheat cultivars ‘Stephens’ and ‘Michigan Amber’. Using
F6 recombinant inbred lines from the ‘Stephens’בMichigan Amber’ cross, we demonstrated that polymorphic bands amplified with
primers based on leucine-rich repeats, nucleotide-binding sites and protein kinase genes, were inherited as single loci. Linkages
between molecular markers and stripe rust resistance genes were detected. This technique provides a new way to develop molecular
markers for assessing the genetic diversity of germplasm based upon potential candidate resistance genes in diverse species.
Received : 5 September 1997 / Accepted : 6 November 1997 |
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Keywords: | Candidate genes Disease resistance genes Germplasm diversity Host-pathogen interaction Molecular marker |
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