Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant |
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Authors: | Nedim Mutlu Filiz Hatice Boyacı Münevver Göçmen Kazım Abak |
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Institution: | Alata Horticultural Research Institute, Erdemli, Mersin, Turkey. nmutlu2@unl.edu |
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Abstract: | Fusarium wilt (Fusarium oxysporum Schlecht. f. sp. melongenae) is a vascular disease of eggplant (Solanum melongena L.). The objectives of this work were (1) to confirm the monogenic inheritance of fusarium wilt resistance in eggplant, (2)
to identify molecular markers linked to this resistance, and (3) to develop SCAR markers from most informative markers. We
report the tagging of the gene for resistance to fusarium wilt (FOM) in eggplant using SRAP, RGA, SRAP-RGA and RAPD markers.
Analysis of segregation data confirmed the monogenic inheritance of resistance. DNA from F2 and BC1 populations of eggplant segregating for fusarium wilt resistance was screened with 2,316 primer combinations to detect polymorphism.
Three markers were linked within 2.6 cM of the gene. The codominant SRAP marker Me8/Em5 and dominant SRAP-RGA marker Em12/GLPL2
were tightly linked to each other and mapped 1.2 cM from the resistance gene, whereas RAPD marker H12 mapped 2.6 cM from the
gene and on the same side as the other two markers. The SRAP marker was converted into two dominant SCAR markers that were
confirmed to be linked to the resistance gene in the F2, BC1 and F2 of BC3 generations of the same cross. These markers provide a starting point for mapping the eggplant FOM resistance gene in eggplant
and for exploring the synteny between solanaceous crops for fusarium wilt resistance genes. The SCAR markers will be useful
for identifying fusarium wilt-resistant genotypes in marker-assisted selection breeding programs using segregating progenies
of the resistant eggplant progenitor used in this study. |
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