QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley |
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Authors: | K Hori T Kobayashi K Sato K Takeda |
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Institution: | (1) Research Institute for Bioresources, Okayama University, Chuo, Kurashiki 710-0046, Japan |
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Abstract: | Fusarium head blight (FHB) resistance was evaluated in a set of recombinant inbred (RI) lines from a cross between Russia
6 (resistant) and H.E.S. 4 (susceptible), which had one of the widest differences of FHB resistance reactions among ca. 5,000
barley germplasm accessions in Okayama University. Field-grown spikes were sampled and inoculated by the ‘cut-spike test’.
Resistance reactions on the parents and RI lines were scored by eleven grades, from resistant (0) to susceptible (10). Quantitative
trait loci (QTL) analysis detected three QTL: two located on the long arm of chromosome 2H, and another on the short arm of
chromosome 5H. A QTL located on chromosome 2H was coincident with the vrs1 locus, which governs inflorescence row type. The other QTL on chromosome 2H was positioned in the vicinity of cleistogamy
locus (cly1 or Cly2) that determines inflorescence opening/closing. Resistant gene analog (RGA) and expressed sequence tag (EST) markers with
homology for disease resistance genes were integrated into the high-density linkage map. Most of these markers were not localized
near the identified resistance QTL, except for one RGA marker (FXLRRfor_XLRRrev170) localized in the vicinity of the cly1/Cly2 locus. Five AFLP markers localized in the vicinity of the identified QTL were sequenced to convert them into sequence tagged
site (STS) markers. Genotyping of each RI line using two AFLP–STS markers and the vrs1 locus indicated that the RI lines with three Russia 6 QTL alleles exhibited the same level of high FHB resistance reactions
as Russia 6. In contrast, RI lines with three susceptible alleles showed reactions close to H.E.S. 4. Therefore, the markers
closely linked to the QTL can be efficiently used for the selection of resistance. |
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