Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA |
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Authors: | J C Huang M Sun |
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Institution: | (1) Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong e-mail: meisun@hkucc.hku.hk, meihk@yahoo.com Fax: +852 2818 8136, CN |
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Abstract: | Genetic diversity and relationships of 40 accessions of Ipomoea, representing ten species of series Batatas, were examined using ISSR markers and restriction-site variation in four non-coding regions of chloroplast DNA. A total of
2071 ISSR fragments were generated with 15 primers in these accessions and, on average, 52 bands per accession were amplified.
Most of the primers contained dinucleotide repeats. The ISSR fragments were highly polymorphic (62.2%) among the 40 accessions
studied. Restriction analysis of chloroplast (cp) DNA revealed 47 informative restriction-site and length mutations. Phylogenetic
analyses of ISSR and cpDNA datasets generally revealed similar relationships at the interspecific level, but the high polymorphism
of ISSRs resulted in a better separation of intraspecific accessions. However, the combined ISSR and cpDNA dataset appeared
to be appropriate in resolving both intra- and interspecific relationships. Of the species examined, I. trifida was found to be the most closely related to cultivated sweetpotato, the hexaploid I.
batatas, while I. ramosissima and I. umbraticola were the most distantly related to I. batatas within the series. Ipomoea triloba, hitherto considered to be one of the ancestors of sweetpotato, was only distantly related to sweetpotato based on ISSR similarity
index.
Received: 4 January 1999 / Accepted: 27 September 1999 |
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Keywords: | Chloroplast DNA Genetic diversity Ipomoeabatatas ISSR RFLP Sweetpotato |
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