Characterization of a rice (Oryza sativa L.) mutant deficient in the heme domain of nitrate reductase |
| |
Authors: | H Hasegawa T Katagiri S Ida O Yatou M Ichii |
| |
Institution: | (1) Research Institute for Advanced Science and Technology, Shinke-cho, Sakai, 593 Osaka, Japan;(2) Faculty of Agriculture, Kagawa University, Miki-cho, 761-07 Kagawa, Japan;(3) Research Institute for Food Science, Kyoto University, Uji, 611 Kyoto, Japan;(4) Institute of Radiation Breeding, NIAR, Ohmiya-machi, 319-22 Ibaraki, Japan |
| |
Abstract: | Summary Biochemical and genetical characterization of a rice nitrate reductase (NR)-deficient mutant, M819, which had been isolated as a chlorate-resistant mutant, was carried out. In M819, leaf NADH-NR activity was found to be about 10% of that of the wild-type cv Norin 8, while NADPH-NR activity was higher than that in the wild-type; FMNH2-NR and MV-NR activities were also 10% of those of the wild type; BPB-NR activity was higher than that of the wild type; and xanthine dehydrogenase activity was revealed to be present in both. These results suggest that the mutant line M819 lacks the functional heme domain of the NADH-NR polypeptide due to a point mutation or a small deletion within the coding region of the structural gene. Chlorate resistance in M819 was transmitted by a single recessive nuclear gene.Abbreviations NR
Nitrate reductase
- NiR
nitrite reductase
- FMNH2
reduced flavin nucleotide
- MV
reduced methyl viologen
- BPB
reduced bromphenol blue
- XDH
xanthine dehydrogenase |
| |
Keywords: | Oryza sativa L Nitrate reductase Deficient mutant Chlorate resistance Heme domain |
本文献已被 SpringerLink 等数据库收录! |
|