Plant regeneration through somatic embryogenesis in callus culture of green bamboo (Bambusa oldhamii Munro) |
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Authors: | Meei ling Yeh Wei chin Chang |
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Institution: | (1) Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China |
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Abstract: | Summary Young inflorescence explants of green bamboo (Bambusa oldhamii Munro) in culture show a high capacity for plant regeneration through somatic embryogenesis. Embryogenic callus was initiated from explants maintained on Murashige and Skoog's medium supplemented with 3 mg/l 2,4-D, 2 mg/l kinetin and a high content (60 g/l) of sucrose. Prolonged culture in the embryoid induction medium or transferral of embryonic callus to auxin-free medium resulted in the continued development and eventual germination of embryoids and establishment of rooted plantlets that were successfully transferred to soil. |
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Keywords: | Somatic embryogenesis Inflorescence callus Plant regeneration Green bamboo Bambusa oldhamii |
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