A mini core subset for capturing diversity and promoting utilization of chickpea genetic resources in crop improvement |
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Authors: | H D Upadhyaya R Ortiz |
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Institution: | (1) Genetic Resources and Enhancement Program, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), P.O. Patancheru 502 324, Andhra Pradesh, India Fax: +91-40-3241239 e-mail: R.Ortiz@CGIAR.ORG, IN |
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Abstract: | A core collection is a chosen subset of large germplasm collection that generally contains about 10% of the total accessions
and represents the genetic variability of entire germplasm collection. The purpose of a core collection is to improve the
use of genetic resources in crop improvement programs. In many crops the number of accessions contained in the genebank are
several thousands, and a core subset consisting of 10% of total accessions would be an unwieldy proposition. In this article
we have suggested a two-stage strategy to select a chickpea mini core subset consisting of only about 1% of the entire collection
held in trust at ICRISAT’s genebank (16,991 accessions). This mini core subset still represents the diversity of the entire
core collection. The first stage involves developing a representative core subset (about 10%) from the entire collection using
all the available information on origin, geographical distribution, and characterization and evaluation data of accessions.
The second stage involves evaluation of the core subset for various morphological, agronomic, and quality traits, and selecting
a further subset of about 10% accessions from the core subset. At both stages standard clustering procedure was used to separate
groups of similar accessions. A mini core subset consisting 211 accessions from 1,956 core subset accessions, using data on
22 morphological and agronomic traits, was selected. Newman- Keuls’ test for means, Levene’s test for variances, the chi-square
test and Wilcoxon’s rank-sum non-parametric test for frequency distribution analysis for different traits indicated that the
variation available in the core collection has been preserved in the mini core subset. The most important phenotypic correlations
which may be under the control of coadapted gene complexes, were also preserved in the mini core. This mini core subset, due
to its drastically reduced size, will prove to be a point of entry to proper exploitation of chickpea genetic resources.
Received: 20 August 2000 / Accepted: 25 September 2000 |
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Keywords: | Agronomic traits Chickpea Diversity Mini core subset Morphological traits |
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