A fertile revertant from petaloid cytoplasmic male-sterile carrot has a rearranged mitochondrial genome |
| |
Authors: | A Chahal H S Sidhu D J Wolyn |
| |
Institution: | (1) Department of Horticultural Sciences, University of Guelph, Guelph, Ont., Canada N1G 2W1 Fax: +1 519-767-0755 E-mail: dwolyn@evbhort.uoguelph.ca, CA |
| |
Abstract: | A spontaneously derived fertile plant was recovered from a petaloid cytoplasmic male-sterile (CMS) carrot inbred line. Genetic
analysis indicated a single nuclear gene was responsible for the restoration to fertility. Within a family segregating for
the nuclear restorer in combination with the sterility-inducing cytoplasm, fertile plants were recovered that could not restore
fertility when crossed to sterile genotypes. Genetic analysis indicated cytoplasmic reversion for fertility, and Southern
analysis, comparing mtDNA organization of the fertile revertant and its CMS progenitor, identified mitochondrial genome rearrangements.
Hybridization of cosmids representing a 108-kb subgenomic circle of the sterile line to DNA of a fertile maintainer and fertile
revertant lines indicated a similar mtDNA organization for these genotypes that was distinct from that of the sterile line.
Six restriction fragments totalling 43.2 kb were common to the fertile maintainer and revertant and absent in the sterile;
other restriction fragments totalling 38.2 kb were present only for the sterile line. Unique fragments of low stoichiometry,
two for the fertile maintainer and three for the revertant, distinguished these lines. The reversion to fertility in the sterile
line could have resulted from the amplification of a mitochondrial submolar genome highly homologous to that found in the
fertile maintainer line.
Received: 4 October 1997/Accepted: 12 December 1997 |
| |
Keywords: | Carrot CMS Fertile revertant Mitochondrial DNA Subgenomic molecules Cosmid mapping |
本文献已被 SpringerLink 等数据库收录! |
|