The green fluorescent protein (GFP) as a vital screenable marker in rice transformation |
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Authors: | P Vain B Worland A Kohli J W Snape P Christou |
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Institution: | (1) John Innes Centre, Colney lane, Norwich NR4 7UH, UK Fax: (44)-1603-502241 E-mail: vain@bbsrc.ac.uk, GB |
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Abstract: | An engineered green fluorescent protein (GFP) from the jellyfish Aequora victoria was used to develop a facile and rapid rice transformation system using particle bombardment of immature embryos. The mgfp4 gene under the control of the 35s Cauliflower Mosaic Virus promoter produced bright-green fluorescence easily detectable and screenable in rice tissue 12–22
days after bombardment. Visual screening of transformed rice tissue, associated with a low level of antibiotic selection,
drastically reduced the quantity of tissue to be handled and the time required for the recovery of transformed plants. GFP
expression was observed in primary transformed rice plants (T0) and their progeny (T1). We describe various techniques to observe GFP in vitro and in vivo. The advantages of this new screenable marker in rice
genetic engineering programmes are discussed.
Received: 6 October 1997 / Accepted: 9 October 1997 |
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Keywords: | Green fluorescent protein Visual screening Transformation Oryza sativa |
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