Genetic analyses supported by molecular methods provide evidence of a new genic (st1) and a new cytoplasmic (st2) male sterility in Allium schoenoprasum L. |
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Authors: | T Engelke T Tatlioglu |
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Institution: | Abteilung Angewandte Genetik, Universit?t Hannover, Herrenh?user Str. 2, D-30419 Hannover, Germany e-mail: thomas.engelke@mbox.genetik.uni-hannover.de Fax: +49-511-762-3608, DE
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Abstract: | Spontaneous mutations leading to male sterility have been described for many different crops and are of great importance to
hybrid breeding, provided that their inheritance is resolved. This paper describes an efficient method to characterise male
sterilities with respect to cytoplasmic factors that might be causally related to them. The differentiation of cytoplasmic
(CMS) and genic (GMS) male sterility is achieved by a specific transfer of nuclear male sterility factors to different cytoplasm
types which have previously been distinguished by means of RFLP analyses using mitochondrial gene probes. The nuclear sterility
factors of Allium schoenoprasum used, st1 and st2, showed a monogenic recessive inheritance in their original cytoplasms. While st1 was expressed in four different cytoplasm types, st2 did not show itself in a cytoplasm type differing from the original. Therefore, the st1-sterility is a GMS, while a cytoplasmic
factor is necessary for the occurrence of st2-sterility. This cytoplasmic factor was verified by a reciprocal cross, and the
CMS system was completed by the selection of maintainer genotypes. Neither of these new sterilities were influenced by high
temperatures or tetracycline. The benefits of a new CMS system to practical breeding and the advantages and disadvantages
of the environmental influences on the expression of male sterility are discussed.
Received: 24 November 1999 / Accepted: 3 December 1999 |
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Keywords: | Cytoplasmic male sterility CMS Genic male sterility GMS Chives Mitochondrial genome |
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