The use of RAPD analysis to classify Triticum accessions |
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Authors: | W Cao G Scoles P Hucl R N Chibbar |
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Institution: | (1) Department of Plant Sciences, Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK., Canada S7N 5A8 Fax: +1-613-759-6559 E-mail: caowen@em.agr.ca, CA;(2) Plant Biotechnology Institute, National Research Council, Saskatoon, SK., Canada S7N 0W9, CA |
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Abstract: | Crop germplasm collections contain a considerable percentage of misclassified accessions which may affect the use of germplasm
for agricultural crop improvement. The objective of this study was to determine if random amplified polymorphic DNA (RAPD)
analysis could be used to reclassify misclassified Triticum accessions. Twelve accessions suspected to be misclassified, based on morphological characters, as either macha or vavilovii
wheat were studied using RAPD and cytological analyses. In the RAPD analysis, a dendrogram, based on Jaccard genetic similarity
coefficients, grouped 5 dicoccum-like, 1 timopheevii-like, and 6 monococcum-like accessions with Triticum dicoccum, T. timopheevii, and T. monococcum accessions, respectively. These results were confirmed by the cytological analysis. A RAPD marker specific to the D genome
was also detected. This study suggests that RAPD analysis can be used to classify germplasm and to distinguish some species
in Triticum.
Received: 12 June 1998 / Accepted: 18 August 1998 |
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Keywords: | Triticum Germplasm RAPD Misclassification Duplication |
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