Genetic relationships among melon breeding lines revealed by RAPD markers and agronomic traits |
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Authors: | E Garcia M Jamilena J I Alvarez T Arnedo J L Oliver R Lozano |
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Institution: | (1) Departamento de Biología Aplicada, Area de Genética, Escuela Politécnica Superior, Universidad de Almería, 04120 Almería, Spain Fax: +34-50-21 54 76 E-mail: rlozano@ualm.es, ES;(2) S & G Semillas, S.A. (Novartis), 04738 Puebla de Vícar, Almería, Spain, ES;(3) Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain, ES |
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Abstract: | RAPD markers and agronomic traits were used to determine the genetic relationships among 32 breeding lines of melon belonging
to seven varietal types. Most of the breeding lines were Galia and Piel de Sapo genotypes, which are currently being used
in breeding programmes to develop new hybrid combinations. A total of 115 polymorphic reliable bands from 43 primers and 24
agronomic traits were scored for genetic distance calculations and cluster analysis. A high concordance between RAPDs and
agronomic traits was observed when genetic relationships among lines were assessed. In addition, RAPD data were highly correlated
with the pedigree information already known for the lines and revealed the existence of two clusters for each varietal type
that comprised the lines sharing similar agronomic features. These groupings were consistent with the development of breeding
programmes trying to generate two separate sets of parental lines for hybrid production. Nevertheless, the performance of
certain hybrids indicated that RAPDs were more suitable markers than agronomic traits in predicting genetic distance among
the breeding lines analysed. The employment of RAPDs as molecular markers both in germplasm management and improvement, as
well as in the selection of parental lines for the development of new hybrid combinations, is discussed.
Received: 25 July 1997 / Accepted: 6 October 1997 |
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Keywords: | Cucumis melo RAPDs Genetic diversity Agronomic markers Germplasm management |
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