A specific oligonucleotide of the 5S rDNA spacer and species-specific elements identify symmetric somatic hybrids between Solanum tuberosum and S. pinnatisectum |
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| Authors: | C Zanke N Borisjuk B Ruoss L Schilde-Rentschler H Ninnemann V Hemleben |
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| Institution: | (1) Division of Biological Sciences, Tucker Hall, University of Missouri, 65211 Columbia, MO, USA;(2) USDA-ARS, Plant Genetics Research Unit, Plant Science Unit, Curtis Hall, University of Missouri, 65211 Columbia, MO, USA |
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| Abstract: | A genetic map of rye, Secale cereale L., chromosome 1R covering 247 cM was constructed utilizing 27 RFLP and four C-band markers, including terminal C-bands. Genetic mapping of C-bands and the centromere, and in situ hybridization of three RFLP clones, allowed for the integration of the genetic and cytological maps. Eight contact points between the genetic and cytological maps revealed variation in the recombination distance to cytological distance ratio ranging between 0.25 and 1.95, a 7.8-fold difference. Recombination was found to be highest in the satellite region of 1RS and lowest in the most distal region of 1RL. |
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| Keywords: | Secale cereale RFLP Cytogenetic mapping C-band In situ hybridization |
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