In situ hybridization to somatic metaphase chromosomes of potato |
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| Authors: | R G F Visser R Hoekstra F R van der Leij L P Pijnacker B Witholt W J Feenstra |
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| Institution: | (1) Department of Genetics, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands;(2) Department of Biochemistry, University of Groningen, Nijenborgh 16, NL-9747 AG Groningen, The Netherlands |
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| Abstract: | Summary An in situ hybridization procedure was developed for mitotic potato chromosomes by using a potato 24S rDNA probe. This repetitive sequence hybridized to the nucleolar organizer region (NOR) of chromosome 2 in 95%–100% of the metaphase plates. Another repetitive sequence (P5), isolated from the interdihaploid potato HH578, gave a  ladderpattern in genomic Southern's of Solanum tuberosum and Solanum phureja, but not in those of Solanum brevidens and two Nicotiana species. This sequence hybridized predominantly on telomeric and centromeric regions of all chromosomes, although chromosomes 7, 8, 10 and 11 were not always labeled clearly. |
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| Keywords: | In situ hybridization Mitotic chromosomes Potato Repetitive sequences |
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