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Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations |
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| Institution: | (1) Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752, USA;(2) Norwegian Forest Research Institute (Skogforsk), Hogskoleveien 12, 1432 As, Norway;(3) Functional Genomics Group, OB120, School of Biological Sciences, University of Bristol, Bristol, BS8 1UG, UK;(4) Institute of Forest Genetics, USDA Forest Service, Pacific Southwest Research Station, Davis, CA 95616, USA;(5) Oak Ridge National Laboratory, MS-6422, P.O. Box 2008, Oak Ridge, TN 37830, USA;(6) School of Forest Resources, Pennsylvania State University, University Park, PA 16802-5805, USA |
| Abstract: | Twenty-two highly variable SSR markers were developed in Douglas-fir Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.Communicated by O. Savolainen |
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