Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance |
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Authors: | C Jung M Kleine E Fischer R G Herrmann |
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Institution: | (1) Botanical Institute of the Ludwig Maximilians University Munich, Menzinger Straße 67, 8000 Munich 19, FRG |
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Abstract: | Summary We have begun to apply techniques for the preparation and anaylsis of large DNA segments from sugar beet (Beta vulgaris) addition lines carrying a mitotically stable chromosome fragment from B. procumbens that confers monogenic resistance to the nematode Heterodera schachtii, with a view towards isolating the resistance gene. DNA probes specific for this chromosome fragment were selected, and various methods for cloning genome-specific fragments, including probes from megabase DNA separated in pulsed-field slab gels, are compared. Probes that display high homology to B. procumbens have been used for hybridization of a representative genomic library and for initial step in mapping the chromosome fragment via pulsed-field gel electrophoresis after restriction with infrequently cutting enzymes. Our data indicate that DNA molecules from the entire chomosome fragment can be separated from protoplast DNA lysates. |
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Keywords: | Sugar beet B procumbens Nematode resistance Pulsed-field gel electrophoresis DNA probes |
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