Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA |
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Authors: | A Tozuka H Fukushi T Hirata M Ohara A Kanazawa T Mikami J Abe Y Shimamoto |
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Institution: | (1) Laboratory of Plant Genetics and Evolution, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Fax: 81-11-706-4933 E-mail: yshimamo@res.agr.hokudai.ac.jp, JP |
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Abstract: | Wild soybean (Glycine soja Sieb. et Zucc.), regarded as the progenitor of cultivated soybean G. max (L.) Merr.], is widely distributed in East Asia. We have collected 1097 G. soja plants from all over Japan and analyzed restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) in
them. Based on the RFLPs detected by gel-blot analysis, using coxII and atp6 as probes, the collected plants were divided into 18 groups. Five mtDNA types accounted for 94% of the plants examined. The
geographic distribution of mtDNA types revealed that, in many regions, wild soybeans grown in Japan consisted of a mixture
of plants with different types of mtDNA, occasionally even within sites. Some of the mtDNA types showed marked geographic
clines among the regions. Additionally, some wild soybeans possessed mtDNA types that were identical to those widely detected
in cultivated soybeans. Our results suggest that the analysis of mtDNA could resolve the maternal lineage among plants of
the genus Glycine subgenus Soja.
Received: 16 June 1997/Accepted: 5 August 1997 |
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Keywords: | Wild soybean Glycine soja RFLP Mitochondrial DNA Geographic distribution |
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