Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population |
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| Authors: | M Maheswaran P K Subudhi S Nandi J C Xu A Parco D C Yang N Huang |
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| Institution: | (1) International Rice Research Institute, P.O. Box 933, Manila, Philippines, XX |
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| Abstract: | We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution
and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs
of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed
over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers
to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with
AFLP markers they will be very useful for marker-assisted backcrossing.
Received: 11 April 1996 / Accepted: 14 June 1996 |
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| Keywords: | Genetic map Marker-aided selection Oryza sativa RFLP markers |
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