RFLP linkage analysis of the Cf-4 and Cf-9 genes for resistance toCladosporium fulvum in tomato |
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| Authors: | P J Balint-Kurti M S Dixon D A Jones K A Norcott J D G Jones |
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| Institution: | (1) Sainsbury Laboratory, John Innes Centre for Plant Science Research, Colney Lane, NR4 7UH Norwich, England, UK;(2) Present address: Laboratory of cellular and developmental biology, NIDDK, National Institutes of Health, 9000 Rockville Pike, 20892 Bethesda, Maryland, USA |
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| Abstract: | Four different populations segregating for one of the two closely linked (possibly allelic) tomato disease resistance genes to the fungusCladosporium fulvum,Cf-4 andCf-9, were generated and analysed for recombination frequencies between theCf-genes and restriction fragment length polymorphism (RFLP) loci. The population consisting of F2 progeny from the interspecific crossLycopersicon esculentum carryingCf-9 ×L. pennellii was identified as the most useful for RFLP mapping of theCf-4/9 locus and an RFLP map around this locus was constructed mainly using this population. The two closest markers identified were CP46, 2.6 cM distal, and a group of 11 markers including TG236, 3.7 cM proximal toCf-4/9. A polymerase chain reaction (PCR)-based procedure for the rapid identification of recombination events between these two markers was developed. The regions of foreign DNA introgression surroundingCf-4 andCf-9 in near-isogenic lines were delimited. |
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| Keywords: | Lycopersicon esculentum Tomato leaf mould Plant disease resistance gene Restriction fragment length polymorphism (RFLP) analysis Genetic linkage |
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