A genetic map of 1,000 SSR and DArT markers in a wide barley cross |
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Authors: | P R Hearnden P J Eckermann G L McMichael M J Hayden J K Eglinton K J Chalmers |
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Institution: | (1) Molecular Plant Breeding Cooperative Research Centre (MPBCRC), PMB 1 Glen Osmond, Adelaide, SA, 5064, Australia;(2) School of Agriculture, Food and Wine, The University of Adelaide, PMB 1 Glen Osmond, Adelaide, SA, 5064, Australia |
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Abstract: | A high-density genetic map was developed from an F1-derived doubled haploid population generated from a cross between cultivated
barley (Hordeum vulgare) and the subspecies H. vulgare ssp. spontaneum. The map comprises 1,000 loci, amplified using 536 SSR (558 loci) and 442 DArT markers. Of the SSRs, 149 markers (153 loci)
were derived from barley ESTs, and 7 from wheat ESTs. A high level of polymorphism (∼70%) was observed, which facilitated
the mapping of 197 SSRs for which genetic assignments had not been previously reported. Comparison with a published composite
map showed a high level of co-linearity and telomeric coverage on all seven chromosomes. This map provides access to previously
unmapped SSRs, improved genome coverage due to the integration of DArT and EST-SSRs and overcomes locus order issues of composite
maps constructed from the alignment of several genetic maps.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Hordeum vulgare spp spontaneum SSR EST DArT Genetic mapping Wild relatives |
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