PCR-based method for identifying the S-genotypes of Japanese pear cultivars |
| |
Authors: | T Ishimizu K Inoue M Shimonaka T Saito O Terai S Norioka |
| |
Institution: | (1) Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan Fax: +816-6879-8619 E-mail: norioka@protein.osaka-u.ac.jp, JP;(2) Tottori Horticultural Experiment Station, 2048 Yurashuku, Daiei, Tottori 689-2221, Japan, JP;(3) Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries, 2-1 Fujimoto, Tsukuba, Ibaraki 305-0852, Japan, JP |
| |
Abstract: | Japanese pear (Pyrus pyrifolia Nakai), a member of the Rosaceae, shows gametophytic self-incompatibility that is controlled by the S-locus. The S-genotype of Japanese pear cultivars is an important factor for crossing and breeding. We report a rapid reliable method to
identify these S-genotypes. It consists of PCR amplification of the S-RNase gene from genomic DNA and subsequent digestion of the PCR fragments
with S-allele-specific restriction endonucleases. Using this method, we determined the unknown S-genotypes of nine Japanese pear cultivars and selected self-compatible varieties from the offspring of the self-compatible
cultivar, ‘Osa-Nijisseiki’.
Received: 8 June 1998 / Accepted: 19 October 1998 |
| |
Keywords: | Japanese pear PCR Rosaceae S-alleles Self-incompatibility |
本文献已被 SpringerLink 等数据库收录! |
|