RFLP-based maps of the homoeologous group-6 chromosomes of wheat and their application in the tagging of Pm12, a powdery mildew resistance gene transferred from Aegilops speltoides to wheat |
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Authors: | J Jia K M Devos S Chao T E Miller S M Reader M D Gale |
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Institution: | (1) John Innes Centre, Norwich Research Park, NR4 7UH Colney, Norwich, UK;(2) Present address: CAAS, Institute of Crop Germplasm Resources, 100081 Beijing, China;(3) Present address: Institute of Botany, Academia Sinica, Rm 114, Nankang, Taipei, Taiwan |
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Abstract: | Genetic maps of the homoeologous group-6 chromosomes of bread wheat, Triticum aestivum, have been constructed spanning 103 cM on 6A, 90 cM on 6B and 124 cM on 6D. These maps were transferred to a Chinese Spring (CS) x line #31 cross to locate a dominant powdery mildew resistance gene, Pm12, introgressed into line #31 from Aegilops speltoides. Pm12 was shown to lie on the short arm of translocation chromosome 6BS-6SS.6SL in line #31, but could not be mapped more precisely due to the lack of recombination between the 6S Ae. speltoides segment and chromosome 6B. Possible strategies to reduce the size of the alien segment, which probably encompasses the complete long arm and more than 82% of the short arm of chromosome 6B, are discussed. |
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Keywords: | Ae speltoides T aestivum RFLP Pm12 Powdery mildew Genetic maps |
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